Senior Cell Biology Scientist

Bethesda, MD

Part Time

National Institutes of Health (NIH)

Experienced

ROLE
We are seeking a Senior Cell Biology Scientist to provide highly specialized scientific and technical support to a National Institutes of Health (NIH) research laboratory within the Epithelial Signaling and Transport Section in Bethesda, MD. The work focuses on epithelial biology, calcium signaling, endoplasmic reticulum–plasma membrane (ER–PM) junction organization, and ion channel regulation, with direct application to ongoing and planned NIH intramural research projects.

This is a senior, part-time, hands-on role that requires immediate proficiency in advanced imaging, molecular biology, and quantitative analysis. The selected candidate will work independently, provide mechanistic insight to complex biological questions, and integrate into an established NIH research environment. This role requires an experienced scientist who can perform the required techniques from day one, with no ramp-up period.

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KEY RESPONSIBILITIES

Design, execute, and analyze independent experimental studies related to epithelial signaling, calcium dynamics, ER–PM junction behavior, and ion channel function
Perform advanced live-cell and high-resolution fluorescence imaging, including TIRF and confocal microscopy, and apply specialized techniques (e.g., FRET, Z-scan, etc.)
Conduct quantitative analysis of imaging and experimental datasets using ImageJ/Fiji, GraphPad Prism, and related analysis tools
Apply advanced molecular and biochemical techniques (e.g., Western blotting, co-immunoprecipitation, surface biotinylation, molecular cloning, PCR, and siRNA-mediated gene knockdown)
Independently troubleshoot complex experimental workflows and interpret mechanistic signaling data
Manage mouse colonies in compliance with institutional and federal animal care requirements (e.g., genotyping, tissue collection, surgical procedures, and euthanasia)
Prepare publication-quality figures, datasets, and written scientific summaries for manuscripts, presentations, and internal scientific meetings
Maintain thorough, accurate, and reproducible electronic laboratory documentation
Train and mentor post-baccalaureate fellows and other laboratory staff in advanced laboratory and imaging techniques

REQUIRED KNOWLEDGE & SKILLS

Expertise in epithelial biology, calcium signaling, ion channel regulation, and/or ER–PM junction dynamics
Proven, independent proficiency with TIRF and confocal microscopy, live-cell imaging, and quantitative image analysis
Ability to work independently with minimal supervision and contribute scientific judgment at a senior level
Strong written communication skills

REQUIRED EXPERIENCE

8+ years of hands-on biomedical research experience in an academic, government, or industry laboratory environment
Extensive experience with molecular and biochemical techniques (e.g., cloning, Western blotting, co-immunoprecipitation, surface biotinylation, and gene manipulation)
Experience contributing to manuscripts, figures, or scientific reports

PREFERRED EXPERIENCE

Experience integrating advanced in vitro or human-relevant model systems into mechanistic biological research
Prior experience supporting or collaborating with NIH or other federal intramural research programs
Record of authorship or substantial contribution to peer-reviewed scientific publications

REQUIRED EDUCATION

Bachelor’s degree in biology or a related life sciences field

PREFFERED EDUCATION

Master’s degree or PhD in biological sciences, bioengineering, or a related discipline
Graduate-level training in regenerative or stem cell biology (e.g., iPSC culture, organoid, or tissue-engineered models)
Completion of a specialized graduate program in regenerative or stem cell biotechnology (e.g., Johns Hopkins, etc.)

LOCATION

Bethesda, MD 20814

TELEWORK

CLEARANCE

U.S. citizenship is required as it supports the U.S. federal government

CLIENT

National Institutes of Health (NIH)

TRAVEL

Travel is not required

WORK HOURS

Part-time (approximately 20 hours/week; 960 hours/year)
Schedule coordinated with the NIH laboratory based on project needs

EMPLOYMENT CLASSIFICATION

Employment Classification Eligibility—Exempt

RELOCATION

Not eligible for relocation benefits

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KMRG, LLC is an Equal Opportunity/Affirmative Action (EEO/AA) employer. All qualified applicants will receive consideration for employment without regard to race, color, creed, religion, sex, gender identity, sexual orientation, ancestry, national origin, age, marital or civil partnership status, mental disability, physical disability, medical condition, pregnancy, maternity status, political affiliation, military or veteran status, genetic information, traits historically associated with race, or any other basis prohibited by federal, state, and/or local law.

Other Considerations. Applicants will be subject to a background investigation. Individual’s primary workstation is located in an office area. The noise level in this environment is low to moderate. Regularly required to sit for extended periods up to 100% of the time; frequently required to move about to access file cabinets and use office equipment such as PC, copier, fax, telephone, cell phone, etc. Occasionally required to reach overhead, bend, and lift objects of up to 10 lbs. Specific vision abilities required by this job include the use of computer monitor screens up to 100% of the time.

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Do you have extensive experience with molecular and biochemical techniques (e.g., cloning, Western blotting, co-immunoprecipitation, surface biotinylation, and gene manipulation)?*

Do you have experience contributing to manuscripts, figures, or scientific reports?*

Do you have expertise in epithelial biology, calcium signaling, ion channel regulation, and/or ER–PM junction dynamics?*

Do you have proven, independent proficiency with TIRF and confocal microscopy, live-cell imaging, and quantitative image analysis?*

Do you have the ability to work independently with minimal supervision and contribute scientific judgment at a senior level?*

Do you have strong written communication skills?*

Are you fully trained in the techniques listed in the key responsibilities and able to perform them independently from day one, with no ramp-up period?*

Years of experience designing and running independent studies in epithelial signaling, calcium dynamics, ER–PM junctions, or ion channel function?*

Years of experience interpreting signaling data and drawing mechanistic conclusions?*

Years of experience with live-cell imaging?*

Years of experience with TIRF (total internal reflection fluorescence) microscopy?*

Years of experience with confocal microscopy?*

Years of experience with specialized imaging methods (e.g., FRET, Z-scan)?*

Years of experience quantifying imaging data using ImageJ/Fiji (or comparable tools)?*

Years of experience performing statistical analysis and figure prep in GraphPad Prism (or comparable tools)?*

Years of experience with key molecular/biochemical methods: Western blotting, co-immunoprecipitation, surface biotinylation, cloning, PCR, and siRNA knockdown?*

Years of experience troubleshooting complex experimental workflows independently?*

Years of experience managing mouse colonies under IACUC (Institutional Animal Care and Use Committee) or equivalent protocols?*

Years of experience with mouse procedures: genotyping, tissue collection, surgery support, and euthanasia?*

Years of experience preparing publication-quality figures and datasets for manuscripts or presentations?*

Years of experience writing scientific summaries for manuscripts, presentations, or internal lab meetings?*

Years of experience maintaining reproducible electronic lab documentation (electronic lab notebooks or similar systems)?*

Years of experience training or mentoring post-baccalaureate fellows, technicians, or junior staff in advanced lab or imaging methods?*

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Senior Cell Biology Scientist

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